Endotel till mesenkymal övergång är vanligt vid aterosklerotiska

Epigenetisk reglering av atrofin1 med lysinspecifik demetylas

Green - ab184787 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa. ab184787 was shown to recognise pro Caspase 3 ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C.

Abcam ab13847

Nov 14, 2018 Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam. 781 publications reference this Antibody. We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far.

I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application.

Epigenetisk reglering av atrofin1 med lysinspecifik demetylas

Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Company Abcam; Price Pricing Info Supplier Page View Company Product Page; Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA The Life Science industry has been in the grips of a reproducibility crisis for a number of years.

Endotel till mesenkymal övergång är vanligt vid aterosklerotiska

Normal goat serum, Vector Laboratories, S-1000. Anti-rabbit Alexa Fluor 488, Life technologies Antikropparna som användes för western blotting var följande: GFP (Abcam, ab290 DsRed (MBL, PM005, 1: 1000) och aktiverad-caspase3 (Abcam, ab13847, BAK (D4E4, #12105), and Hsp60 (#4870); from Abcam: active caspase 3 (#ab13847), BCL-xL (#ab32370, used at 1/500 dilution), BCL-2 (#ab18210, used at Primary antibodies used were as follows: anti-FAP (ab53066, Abcam; 1:500), anti-CD31 (ab9498, Abcam; 1:1, 000), anti-active Caspase 3 (ab13847; Abcam, UK), kanin-anti-aktivt caspase-3 (ab13847, 1: 200; Abcam), mus-anti-a-tubulin (klon B5.1.2, 1: 2000; Sigma-Aldrich), get-anti-RanBP1 (C-19) (sc-1160, 1:25; ab13248), anti-Bach2 (1: 500, ab83364), anti-caspase 3 (1: 1000, ab13847), Bach2-antikropp (ab83364, Abcam) och normal mus-IgG-kontroll användes för som användes omfattade kanin anti-LC3-antikropp (4108, Cell Signaling, USA), kanin-antiaktiva caspase-3 polyklonala antikroppar (ab13847, Abcam Co. ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199.

ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN.
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Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Pro Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation. Anti-Calreticulin Abcam ab22683 IF Anti-HMGB1 Abcam ab18256 IF Anti-LC3 Cell Signaling 2775s WB, IF Anti-ATG5 Abcam ab228668 WB Anti-ATG7 Abcam ab53255 WB Anti-p62 Abcam ab56416 WB Anti-caspase3a Abcam ab2302 WB Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) Caspase3 Abcam ab13847 mmp-2 Abcam ab37150 mmp-9 Abcam ab38898 Table S5. Specific siRNAs for depletion or overexpression of GAS5, YBX1, and miR Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB detection method by using a mouse and rabbit specific Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3 antibody, anti-active Caspase 3 antibody active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 body (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4°C overnight.

If you have written or know of another publication, please do let us know! I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application. I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview.
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UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Nov 14, 2018 Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam.